Immunohistochemical analysis of Ninjurin 1 in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in Ninjurin 1 monoclonal antibody (Product # MA5-24365) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells.